Development of Simple Sequence Repeat Markers from Bacterial Artificial Chromosomes without Subcloning
نویسندگان
چکیده
منابع مشابه
Development of simple sequence repeat markers from bacterial artificial chromosomes without subcloning.
Simple sequence repeats (SSRs) were isolated from pearl millet bacterial artificial clones (BACs) without any subcloning steps. SSR sequences were targeted using 3' end-anchored SSR primers. Flanking sequences were isolated by suppression PCR. In this pilot study, 25 SSR markers have been developed from 40 BAC pools, comprising a total of 384 clones. This novel way to develop new markers has th...
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Tomato (Solanum lycopersicum L.) is an important vegetable crop and acts as model plant for fruit development studies. Besides that, post-harvest damage is a devastating phenomenon often associated with ripening process in tomato which in turn leads to greater yield loss. Understanding the genetics, molecular and biochemical pathways is the key to overcome the existing situation. In th...
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Comparing different methods of estimating the genetic diversity could define their usefulness in plant breeding programs. In this study, a total of 18 morphological traits and 20 simple sequence repeat (SSR) loci were used to study the morphological and genetic diversity among 20 maize hybrids selected from different countries, and to classify the hybrids into groups based on molecular profiles...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2001
ISSN: 0736-6205,1940-9818
DOI: 10.2144/01312st08